Gibco™ Advanced DMEM, 500 mL

Gibco™ Advanced DMEM, 500 mL 

Advanced DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM, with no adaptation, include MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero.

Features

• With High glucose, Non Essential Amino Acids, Sodium pyruvate & Phenol Red.
• Without L-glutamine.
• Advanced DMEM is unique from other media due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX® I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.
• Many cell lines do not require adaptation to this media.

Using Advanced DMEM

• Advanced DMEM requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement.
• The FBS concentration must be optimized for each cell line to obtain maximum serum reduction.
• Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.