Applied Biosystems™ ELISA-Light™ Immunoassay System with CDP-Star™ and Emerald-II™ Substrate/Enhancer Solution

Applied Biosystems™ ELISA-Light™ Immunoassay System with CDP-Star™ and Emerald-II™ Substrate/Enhancer Solution

The ELISA-Light™ Immunoassay System with CDP-Star® and Emerald-II™ Substrate⁄Enhancer Solution is used for ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.

Features

• Heterogeneous assay format increases sensitivity and dynamic range and reduces interference by test compounds in the detection portion of the assay (as compared with homogeneous assay formats)

• Chemiluminescent immunoassay detection allows greater sensitivity and improved dynamic range as compared to radioimmunoassays and immunoassays that employ colorimetric or fluorescent detection

• High sensitivity and wide dynamic range permit sensitive detection with fewer assays, because sample dilution to fit the dynamic range of the assay is unnecessary

• A variety of ready-to-use substrate formulations lets the user precisely optimize signal intensity to the exact requirements of an assay

• The kinetics of the glow-assay provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used

• Chemiluminescent immunoassay detection provides versatile applications such as sandwich immunoassays, competitive immunoassays, and DNA probe capture assays

• Non-radioactive immunoassays eliminate concerns over use of radioisotopes

Extend the Limits of Detection

The ELISA-Light™ system offers an excellent combination of high sensitivity and throughput for assays measured in a luminometer. The ELISA-Light™ system significantly extends the limits of detection of most immunoassays previously performed with colorimetric and isotopic methods.

Multiple Substrate⁄Enhancer Formulations

The kit contains reagents optimized for chemiluminescent ELISAs employing alkaline phosphatase labels and is available with a choice of ready-to-use chemiluminescent substrate formulations containing CSPD® or CDP-Star® substrate with Sapphire-II™ or Emerald-II™ luminescence enhancers. Multiple substrate⁄enhancer formulations provide options that let the user optimize specific assays or luminometers. The specific formulation that will provide the highest sensitivity for a specific assay can vary. Therefore, an ELISA sampler kit containing all four substrate⁄enhancer formulations is available to facilitate assay development.

• Product Line: ELISA-Light™
• Product Type: Immunoassay System with Substrate/Enhancer Solution
• Contents & Storage: This kit contains enough reagent for 1000 assays. Store at 4°C.

Contents

• Microplate assays 
• I-Block™ Reagent 
• 10X Assay Buffer 1
• CSPD® Substrate/ Sapphire-II Enhancer RTU 
• CSPD® Substrate/ Emerald-II Enhancer RTU 
• CDP-Star® Substrate/ Sapphire-II™ Enhancer RTU 
• CDP-Star® Substrate/ Emerald-II™ Enhancer RTU