Applied Biosystems™ MeltDoctor™ HRM Reagent Kit

Applied Biosystems™ MeltDoctor™ HRM Reagent Kit

Applied Biosystems™ MeltDoctor™ HRM Reagent Kit provides individually packaged PCR components and the MeltDoctor™ HRM Dye required for high resolution melting analysis. High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences.

For use with: 7500 Fast System, 7900HT Fast System

Features

• AmpliTaq Gold™ 360 DNA Polymerase which, when combined with AmpliTaq Gold™ 360 Buffer and 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq Gold™ 360 DNA Polymerase delivers 360° coverage for a full range of targets.
• GeneAmp™ dNTP Blend
• MeltDoctor™ HRM Dye, a stabilized form of the fluorescent SYTO™ 9 double-stranded nucleic acid stain developed by Molecular Probes

Contents

• AmpliTaq Gold™ 360 DNA Polymerase (2 x 50 µL, each at 5 U/µL), AmpliTaq Gold™ 360 Buffer, 360 GC Enhancer, GeneAmp™ dNTP Blend, and MeltDoctor™ HRM Dye (20x). The kit provides sufficient materials for 250 20-µL reactions when using 2 U AmpliTaq Gold 360 DNA Polymerase per reaction.