Gibco™ Expi293™ Expression System Kit

Gibco™ Expi293™ Expression System Kit

The Expi293 Expression System is a complete solution for rapid, high-yield protein production from mammalian 293 cells. It is based on integrated components including high-density Expi293F cells in Expi293 Expression Medium and the cationic lipid-based ExpiFectamine 293 transfection reagent in combination with specialized transfection enhancers.

Features

• Up to 1 g/L of protein; 2- to 10-fold higher protein yields than previous generation transient expression systems such as the FreeStyle 293 Expression System
• Rapid production of recombinant proteins in 5–7 days
• High density cultures of Expi293F cells that result in more expressing cells per milliliter of culture
• Native folding and mammalian post-translational modifications of expressed proteins
• Easy, robust culture and transfection protocols
• Scalable expression from <1 mL to >10 L

Contents

• Frozen Expi293F cells: 2 vials
• Expi293 Expression Medium: 1 bottle 1000 mL
• ExpiFectamine 293 transfection kit for 1 L of culture: 1 Kit
• Antibody Expressing Positive Control Vector: 1 vial 
• Opti-MEM Reduced Serum Medium: 1 bottle 100 mL