Invitrogen™ Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit
On demand
Order number :
C10456
Invitrogen™ Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit
The Click-iT® Plus OPP Alexa Fluor® 488 Protein Synthesis Assay Kit provides a fast, sensitive, and non-radioactive method for the detection of protein synthesis using fluorescence microscopy or high-content imaging. In this assay O-propargyl-puromycin (OPP) is efficiently incorporated into newly translated proteins in complete methionine-containing media and fluorescently labeled with a bright, photostable Alexa Fluor® dye in a fast, highly-specific, and mild click reaction.
Features
- No media change required—works in complete, methionine-containing media, no need to remove cell media
- Multiplex-enabled—Click-iT® Plus technology retains signal from GFP and binding of fluorescent-conjugated phalloidins
- Non-radioactive—an alternative to the traditional 35S-methionine methods
- Works in vivo—published results demonstrate use in vivo for determination of protein translation
Contents
- Click-iT™ OPP Reagent, Alexa Fluor™ dye picolyl azide, Click-iT™ OPP Reaction Buffer, Copper Protectant, Click-iT™ Reaction Buffer Additive, Click-iT™ Reaction Rise Buffer, and NuclearMask™ Blue Stain.
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