Invitrogen™ PureLink™ HiPure Plasmid Midiprep Kit, 50 Preps

Invitrogen™ PureLink™ HiPure Plasmid Midiprep Kit, 50 Preps

The PureLink® HiPure Plasmid Midiprep Kit is designed to isolate transfection-grade plasmid DNA from E. coli. The Midiprep Kit protocol will typically yield 100–350 µg plasmid DNA from 15–25 mL bacterial culture, at a purity that is comparable to that achieved by two passes through cesium chloride gradients.
Anion-exchange chromatography for plasmid purification
The PureLink™ HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to a level equivalent to two passes through CsCl gradients. The resin combines excellent capacity with a fast flow rate, high resolution, high yield, and efficient endotoxin removal. Plasmid preparations can typically be completed in less than 2 hours.


Fratures

• Labor and time saving—no need for extra steps to remove endotoxin and other contaminants
• High-purity, low-endotoxin product—pure enough for mammalian cell transfection
• Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAs
• Sample Type: Bacterial Culture
• Elution Volume: 5 mL
• Plasmid: <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
• Prep Scale: 100-200 µg (Medium-Scale) Plasmid DNA
• Purity: Transfection Grade
• Target: BAC DNA, Plasmid DNA

Contents

• Resuspension Buffer (R3): 200 mL
• RNase A: 1.5 mL
• Lysis Buffer (L7): 200 mL
• Precipitation Buffer (N3): 200 mL
• Equilibration Buffer (EQ1): 2 × 250 mL
• Wash Buffer (W8): 2 × 500 mL
• Elution Buffer (E4): 250 mL
• TE Buffer: 30 mL 
• HiPure Columns: 50
• Column Holders: 10