Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 chemically competent E. coli cells, 50 Reactions
On demand
Brand origin :
United States
Expiry time :
On demand
Delivery time :
1 Week/s
Delivery cost :
On demand
Unit size :
50 Reactions
Order number :
K450040
Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 chemically competent E. coli cells, 50 Reactions
TOPO® TA Cloning® Kits for Subcloning provide a highly efficient, 5-minute, one-step cloning strategy ('TOPO® cloning') for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning. Each kit uses the pCR™ 2.1-TOPO® TA vector with convenient restriction sites for subcloning. The cloning kits are available with a variety of competent cells, or without competent cells, depending upon your needs and budget.
Features
- Fast and easy—go from PCR to clones in just 3 steps and in as little as 5 minutes hands-on time
- Efficient—obtain up to 95% clones with correct insert
- Proven—reliable performance for over a decade with over 4,000 citations
- Simple—no ligase, post-PCR procedures, or PCR primers containing specific sequences are required
Contents
Box 1:
- Topoisomerase I-activated pCR™2.1-TOPO™ vector
- PCR buffer
- Salt solution
- dNTPs
- Control template
- M13 forward and reverse primers
- Control PCR primers
- Sterile water
Box 2:
- One Shot™ Chemically Competent or Electrocomp™ E. coli
- S.O.C. medium
- Supercoiled pUC19 control plasmid
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