One-Step Real Time RT-PCR for Detection of COVID-19 Corona Virus, 50rxn
The kit is intended for the in vitro detection and quantification of COVID-19 RNA in human nasopharyngeal and oropharyngeal swab or sputum specimens utilizing 1-step Reverse Transcriptase-Polymerase Chain Reaction (1-step RT-PCR) and TaqMan probe technology. The COVID-19 Primers and Probes contain primer pairs and TaqMan probes specific for COVID-19 target/standard. The COVID-19 RNA signal is detected in the HEX channel. The human 5S RNA is used as the IC (HEX channel). In each run, the P (positive control) and N (negative control) are also included.
The COVID-19 kit P (positive control) is a non-infectious synthetic DNA molecule containing COVID-19 ORF1b gene sequence. The COVID-19 kit N (negative control) is the dd H2O contain no template. The sample is considered as COVID-19 positive (+) if the unknown sample Ct is faster than that of the N.
- Primers and Taqman probe mix.
- 2x RT-PCR buffer.
- Enzyme mix.
- dd H2O.
- P (E7 copies/5 ul).