Thermo Scientific™ 1-Step Human Coupled IVT Kit - DNA, 8 Reactions

Thermo Scientific™ 1-Step Human Coupled IVT Kit - DNA, 8 Reactions

Thermo Scientific 1-Step Human In Vitro Protein Expression Kits enable the translation and post-transcriptional modification of full-length proteins from mRNA or plasmid templates with yields of up to 100 µg/mL per reaction.

The Human IVT Kits are unique HeLa cell lysate-based protein expression systems for in vitro translation or coupled transcription/translation reactions. Protein expression is performed in a single 90-minute reaction that can be extended for up to 6 hours with continued protein production up to 100 µg/mL when combined with the optimized pT7CFE1 Expression Vector. The Human IVT Kits can express functional enzymes, phosphoproteins, glycoproteins and membrane proteins for immediate use in studying protein interactions, performing rapid mutational analysis and measuring activity.

Features

• Functional—uses the human translational machinery to express active proteins
• Convenient—perform transcription and translation in a single step
• High performance—greater yields compared to rabbit reticulocyte in vitro translation
• Reliable—express proteins that fail in rabbit reticulocyte systems

Contents

Sufficient For: 8 reactions of 25 µL each

• HeLa Lysate, 110 µL
• Accessory Proteins, 25 µL
• Reaction Mix, 40 µL
• Positive Control DNA: pCFE-GFP, 10 µg
• pT7CFE1-CHis, 10 µg
• Nuclease-Free Water, 1.5 mL