Thermo Scientific™ EZ-Link™ PFP-Biotin

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Order number : 21218
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Thermo Scientific™ EZ-Link™ PFP-Biotin

Thermo Scientific EZ-Link PFP-biotin is a pentafluorophenyl-ester biotinylation reagent that has similar properties to NHS-biotin but is more reactive and will target both primary and secondary amines for biotinylation.

PFP-Biotin is an amine-reactive biotinylation reagent based on activation as as a pentafluorophenyl ester (PFP ester) rather than the usual N-hydroxysuccinimide (NHS) ester. The compound has properties similar to those of NHS-Biotin (Part No. 20217) but exhibits greater reactivity toward amino groups. Unlike NHS-Biotin, PFP-Biotin will react with both secondary amines and primary amines, enabling it to be used to label nucleic acids as well as proteins. The reaction occurs spontaneously at pH 7 to 9.


  • Molecular labeling—biotinylate proteins and nucleic acids for avidin-biotin methods
  • Membrane-permeable—can be used to label inside cells (intracellular)
  • Amine-reactive—reacts with primary and secondary amines of proteins and other molecules (unlike typical NHS reagents, which react with primary amines only)
  • Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
  • Solubility—must be dissolved in DMSO or DMF before further dilution in aqueous buffers
  • Very short—spacer arm (total length added to target) is 9.6 angstroms; it consists of the native biotin valeric acid group only


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