Thermo Scientific™ pT7CFE1-NHis-GST-CHA Vector for Mammalian Cell-Free Protein Expression
On demand
Order number :
88871
Thermo Scientific™ pT7CFE1-NHis-GST-CHA Vector for Mammalian Cell-Free Protein Expression
Thermo Scientific pT7CFE1-NHis-GST-CHA is a cloning plasmid optimized to use with the Thermo Scientific 1-Step Human In Vitro Protein Expression System for in vitro translation (IVT) of tagged fusion proteins. pT7CFE1-NHis-GST-CHA Vector is available with tandem affinity tags, 9xHis and GST at the N-terminus and HA tag at the C-terminus, to facilitate protein purification and detection. pT7CFE1-NHis-GST-CHA also has a cleavable tag, HRV 3C, available on the N-terminus.
Features
- EMCV IRES at the 5' UTR promotes high-level translation of mRNAs
- MCS accommodates gene insertion via ten different restriction sites: Msc1, Nde1, BamH1, EcoR1, EcoRV, Pac1, Pst1, Sac1, Sal1, Not1 and Xho1
- Poly A sequence in the 3' region promotes mRNA stabilization and protection from nucleases
- T7 terminator ensures synthesis of accurate size mRNA transcripts
- Plasmid linearization may be accomplished with restriction sites between Poly A sequence and the T7 terminator region
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