Thermo Scientific™ GeneJET Plasmid Midiprep Kit, 100 Preps

Thermo Scientific™ GeneJET Plasmid Midiprep Kit, 100 Preps

Thermo Scientific GeneJET Plasmid Midiprep Kit, sufficient for 100 preps, is designed for large scale isolation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200 µg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Features

• Efficient—high yields up to 200 µg of high quality plasmid DNA from 50 mL of bacterial culture
• High concentration of DNA purified—> 400 ng/ µL
• Convenient—spin column format, centrifuge or vacuum protocols
• Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Contents

• Resuspension Solution:  240 mL
• Lysis Solution:  240 mL
• Neutralization Solution:  240 mL
• Endotoxin Binding Reagent:  60 mL
• Wash Solution I (concentrated):  360 mL
• Wash Solution II (concentrated):   2 × 180 mL
• RNase A Solution:   8 × 1.2 mL
• Elution Buffer (10 mM Tris-HCl, pH 8.5):   60 mL
• GeneJET Midi Purification Columns pre-assembled with collection tubes (15 mL):  100
• Collection tubes (15 mL):  100