Vitassay qPCR SARS-CoV-2

Vitassay qPCR SARS-CoV-2

The viral RNA extracted is transcribed into cDNA using a specific primer by reverse transcription step followed immediately in the same well by polymerase chain reaction. The presence of SARS-CoV-2 is detected by an increase in observed fluorescence during the reaction upon hydrolysis of the fluorescent probe. Vitassay qPCR SARS-CoV-2 is a ready-to used test which contains in each well all the necessary reagents for real-time PCR assay in a stabilized format. In addition, an internal control allows the detection of a possible reaction inhibition.

Analytical sensitivity: The analytical sensitivity was determined by analysis of 10-fold dilution series of SARS-CoV-2 template ranging from 107 to 101 copies/rxn.

Compatible RT-PCR Instrument

• Cobas Z480 (Roche)
• 7500 Fast Real-Time PCR System (Applied Biosystems).
• StepOne™ Real-Time PCR System (Applied Biosystems).
• CFX96 ™ Real-Time PCR Detection System (Bio-Rad).
• AriaMx Real-Time PCR System (Agilent Technologies).
• DTlite Real-Time PCR System (DNA-Technology).
• DTPrime Real Time Detection Thermal Cycler (DNA-Technology).
• Rotor-Gene® Q (Qiagen).
• SmartCycler® (Cepheid).

Contents

• SARS-Cov-2 strips.
• SARS-Cov-2 Positive Control.
• PCR grade water.
• Rehydration Buffer.
• Negative Control.
• Optical caps.