2X Ampli-Optimization Kit
On demand2X Ampli-Optimization Kit
The 2X Ampli-Optimization Kit is designed to provide an easy and convenient way to optimize reaction conditions specific for your amplicon. This kit contains the 2X Taq Master Mix with a range of MgCl2 concentrations (2.0-7.0mM). Other reaction components included in 2X Taq Master Mix such as dNTPs, reaction buffer and Taq DNA Polymerase are provided at the optimized concentration. This kit enables the user to determine the specific MgCl2 concentration for their amplification reaction.
Contents
The kit contains 6 tubes of 2X Taq Master Mix with different MgCl2 concentrations. Each 2X Taq Master Mix consists of Taq DNA Polymerase (0.05U/µl), 0.4mM dNTPs and 2X ViBuffer A [20mM Tris-HCl (pH9.1 at 20°C), 100mM KCl, 0.02% Triton X-100].
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