Alpha Reverse Transcriptase, 10000 U

Alpha Reverse Transcriptase, 10000 U 

MGI Alpha Reverse Transcriptase (MGI RT) is an improved enzyme that was developed through in vitro molecular evolution of M-MuLV RT. MGI RT is designed with significantly improved thermostability, enhanced processivity, increased affinity and diminished RNase H activity to enhance consistency and efficiency in cDNA synthesis for RT-PCR and RT-qPCR.  

These features support higher overall cDNA yields with a variety of templates and improved synthesis from templates with complex secondary structures. In RT-qPCR applications, MGI RT also enables high efficiency synthesis over a wide range of input template amounts providing sensitive and accurate quantification of cDNA. 

Features

• Great thermostability, Up to 60℃.
• Excellent sensitivity, cDNA synthesis from total RNA as low as 10 pg; support a wide range of input template (10 pg-1 ug)
• High cDNA yield, The enzyme ensures high yields of full-length cDNA products up to 11 kb.
• Application: First strand cDNA synthesis for RT-PCR and RT-qPCR, RNA library construction, Single cell RNA library construction, Synthesis of full length cDNA for cloning and expression, Generation of labeled cDNA probes for microarrays, Analysis of RNA by primer extension. 
• Low RNase H activity, Decreased by 80.8%, which helps to prevent premature degradation of RNA.
• Reaction Temperature, 42-50℃.
• Optimum Temperature, 42℃.
• Purity by SDS-PAGE, ≥90% pure as determined by SDS-PAGE analysis through Coomassie Blue detection.
• Stability, Stored at -20 °C Avoid repeated freezing and thawing.

Contents

• Alpha Reverse Transcriptase (200U/uL): 10000 U 
• 5 × First Strand Buffer: 250 µL 
• 0.1 M DTT: 120 µL