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Applied Biosystems™ Arcturus™ Paradise™ PLUS Whole Transcript Reverse Transcription Kit (WT-RT), 12 samples

On demand
Order number : KIT0315
(Ex-work price)
$ On demand

Applied Biosystems™ Arcturus™ Paradise™ PLUS Whole Transcript Reverse Transcription Kit (WT-RT), 12 samples


The ARCTURUS® Paradise® PLUS Whole Transcript RT (WT-RT) Reagent System provides an efficient and reliable solution for purifying RNA from archived Formalin-Fixed Paraffin-Embedded (FFPE) tissue samples followed by reverse transcription of whole transcript for use in real-time PCR. FFPE samples introduce unique challenges for gene expression profiling and gene expression quantitation, including chemical modification and fragmentation of RNA molecules. Storage of FFPE samples adds to these challenges through increased RNA degradation over time. The Paradise® PLUS WT-RT Reagent System was specifically developed and specially optimized to provide high-quality RNA and robust reverse transcription (RT) from highly fragmented RNA obtained from archived FFPE samples over 20 years old


Features

  • Simple workflow—minimal sample handling reduces chance of contamination 
  • Efficient RNA extraction and isolation—saves time and minimizes sample loss
  • Optimized RT reactions—generate cDNA representing the entire length of the transcript
  • Accurate transcript analysis—results represent high, medium, and low-abundance expressing genes
  • For Use With (Equipment): ArcturusXT™ LCM Instrument
  • Storage:  Variable storage conditions
  • Workflow Step: RNA Extraction, Reverse Transcription
  • Sample Type: Tissue (FFPE)


Contents

  • Paradise RNA Isolation kit (RNA extraction and purification reagents, purification columns)
  • cDNA synthesis kit


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