Applied Biosystems™ Axiom™ Apple Genotyping Array

Applied Biosystems™ Axiom™ Apple Genotyping Array

The 96-format array includes markers identified using whole-genome sequence data from 63 Malus domestica cultivars and two double haploid accessions. Table 1 lists the number of cultivars and corresponding country of origin used in the SNP discovery process.

Axiom Apple Genotyping Array includes 480,000 markers and together with Axiom Analysis Suite software overcomes the genotyping challenges associated with polyploidy, rapid linkage disequilibrium (LD) decay, and high polymorphism observed in the apple genome.

Features

• Very high diversity
• Includes markers discovered in 63 worldwide M. domestica cultivars
• High resolution to address rapid decay of LD - 487,249 markers on the array

   - Bias towards common variants with minor allele frequency (MAF) >0.05

   - Includes 21,463 previously validated markers: 19,990 markers from an existing in market 20K Fruitbreedomics apple array and 1,473 markers identified uing genotyping-by-sequencing (GBS)

• Absence of paralogous variants through the use of double haploid accessions in SNP discovery