Applied Biosystems™ cAMP-Screen Direct™ Cyclic AMP Immunoassay System, 960 Tests

Applied Biosystems™ cAMP-Screen Direct™ Cyclic AMP Immunoassay System, 960 Tests

The cAMP-Screen Direct® 96-well Cyclic AMP Immunoassay System enables ultrasensitive determination of cyclic AMP (cAMP) levels in cell lysates. This competitive chemiluminescent immunoassay is formatted with maximum flexibility to permit either manual reagent additions or automated high-throughput processing. The cAMP assay utilizes the highly sensitive chemiluminescent CSPD® Substrate with Sapphire-II™ Enhancer that is triggered by an enzyme conjugate composed of cAMP bound to alkaline phosphatase (cAMP-AP). The chemiluminescent substrate/enhancer formulation is a ready-to-use reagent that generates a sustained-glow light emission from 30 minutes after addition. This assay is mainly used in secondary screening and pre-clinical research, where sensitivity and no false positives are essential.

• The highest sensitivity of any commercially available cAMP assay

• Hours of read time with little or no degradation of the signal

• A simple protocol with cells grown directly on the assay plate

• Useful for a wide range of receptor activation studies

Features

• Product Line: cAMP-Screen Direct™
• Target Kit Named: Cyclic AMP
• Contents & Storage: 1 kit, store at 2°C to 8 °C

Content

• Microplate assays per kit, 960 
• Pre-coated Microplates (tissue culture-treated white with clear bottoms), 10 plates 
• Assay/Lysis Buffer, 2 x 65 mL 
• cAMP Standard, 2 x 5 mL 
• Anti-cAMP Antibody, 2 x 35 mL 
• cAMP-AP Conjugate, 2 x 250 μL 
• Conjugate Dilution Buffer, 2 x 25 mL 
• Wash Buffer, 2 L (1X) 
• CSPD/Sapphire-II RTU Substrate/Enhancer Solution, 2 x 65 mL 
• Plate Backing Sheets, 10 sheets