Applied Biosystems™ RNA UltraSense™ One-Step Quantitative RT-PCR System

Applied Biosystems™ RNA UltraSense™ One-Step Quantitative RT-PCR System

The RNA UltraSense™ One-Step Quantitative RT-PCR System is specially designed for amplification and real-time quantification of ultra-low abundance transcripts and RNA viruses. The optimized, ultra-concentrated system combines SuperScript™ III RT and Platinum™ Taq DNA Polymerase to provide greater priming specificity, higher product yields, and detection over a broad dynamic range. The high concentration formulation provides great flexibility with low concentration / high volume RNA samples, and improves performance with targets rich in secondary structure. As little as 25 pg/µl total RNA can be detected with a PCR efficiency of 97%.

Features

• SuperScript™ III RT for higher temperature cDNA synthesis (up to 60°C) for greater success with RNA secondary structure
• Platinum™ Taq DNA Polymerase with hot-start technology for improved specificity
• Optimized performance with either LUX™ Fluorogenic Primers or dual-labeled fluorogenic probes
• Proprietary, ultra-concentrated buffer for addition of large sample volumes (up to 70%) and success with RNA secondary structure
• Validated performance on multiple real-time instrument platforms
• One-step format for speed, convenience, and less reaction-to-reaction variability

Contents

• RNA UltraSense™ Enzyme Mix (includes SuperScript™ III RT, Platinum™ Taq DNA Polymerase, and RNaseOUT™ Ribonuclease Inhibitor): 250 µl
• RNA UltraSense™ 5X Reaction Mix: 2 x 1 ml
• 20X Bovine Serum Albumin (BSA), Ultrapure Molecular Biology Grade (5 mg/ml): 300 µl
• 50-mM magnesium sulfate (MgSO4 ): 1 ml
• ROX Reference Dye: 100 µl