Applied Biosystems™ TaqMan™ Array, Human Stem Cell Pluripotency, Fast 96-well

Applied Biosystems™ TaqMan™ Array, Human Stem Cell Pluripotency, Fast 96-well

These 96-well plates are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment. The panel of assays in the TaqMan® Array 96-well Human Stem Cell Pluripotency Plate was selected in cooperation with the International Stem Cell Initiative (2007 Nature Biotechnology. 25:803-816). The assays target genes which are validated as markers for the characterization of human embryonic stem cell identity, and which assess variations between embryonic stem cell isolates. Genes are included based on the following criteria: 1) Expression in human embryonal carcinoma cells, primordial germ cells or human embryonic stem cells; 2) Known role in maintenance of pluripotency in human embryonic stem cells; 3) Identification in microarray-based experiments as being correlated with the stem cell state. In order to identify cells which have left the pluripotent state, we have also included genes whose expression is characteristic of specific differentiation lineages in the post implantation embryo and mature tissues.

Internal Probe Modification: MGB (Minor Groove Binder) (3'), Non-Fluorescent Quencher (3'), FAM (5')

Contents

• Each TaqMan™ Array Plate well contains a single TaqMan™ Assay for one 10 µL reaction