Applied Biosystems™ TaqMan™ Copy Number Reference Assay, human, TERT, 3000 Reactions

Applied Biosystems™ TaqMan™ Copy Number Reference Assay, human, TERT, 3000 Reactions

Human TaqMan® Copy Number Reference Assays are run with human TaqMan® Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations (CNVs) and smaller regions in the human genome. Two options for genes that can be used as endogenous reference genes in humans are offered: RNase P and TERT. TaqMan® Copy Number Reference Assays are designed to unique genomic sequences in the reference genome assembly (Build 37/hg19) and are required for relative quantitation of copy number targets.

TaqMan® Copy Number Assays quantitate the gene of interest and normalize to an endogenous reference gene known to be present in two copies in a diploid genome. Please note that TaqMan® Copy Number Reference Assays are species specific.

Contents

• Contains 4 tubes (750 µl each) of 20X pre-formulated assay with reference sequence-specific forward and reverse primers and 1 VIC™ dye-labeled TAMRA™ probe. The tube contains enough assay for 3000 20-µl reactions or 6000 10-µl reactions in 96-well format plates. Assays should be stored at -15°C to -25°C in the dark. Minimize freeze-thaw cycles.