Applied Biosystems™ TaqMan™ Mutation Detection IPC Reagent Kit

Applied Biosystems™ TaqMan™ Mutation Detection IPC Reagent Kit

The TaqMan™ Mutation Detection IPC Reagent Kit is a set of optional internal positive control reagents that can be duplexed with any TaqMan™ Mutation Detection assay to provide a positive PCR control result. The IPC reagents can distinguish a mutation target negative result from a PCR failure result. The TaqMan™ Mutation Detection IPC Reagent Kit is preoptimized for use with the TaqMan™ Mutation Detection Assays. The IPC reagents can be duplexed with any TaqMan™ Mutation Detection Assay and gDNA sample, without compromising the mutation detection assay reaction efficiency. This feature allows the mutation detection assays to detect low copies of mutated DNA in the presence of a robust PCR-positive signal from the IPC reagents.

For use with: 7500 Fast System, 7500 System, StepOnePlus™, ViiA™ 7 System

Features

• Control Template: non-human DNA
• Control Type: Positive Control 

Contents

• 50X Exogenous IPC template DNA (200 µL): A synthetic template that contains the exogenous, non-human DNA target for the assay
• 10X Exogenous IPC Mix (1 mL): A primer-limited TaqMan™ assay designed to amplify an exogenous, non-human DNA target; the mix includes: Two PCR primers, A TAMRA™ probe (VIC™ dye-labeled)