Applied Biosystems™ TaqMan™ PreAmp Master Mix, 40 reactions
On demand
Order number :
4391128
Applied Biosystems™ TaqMan™ PreAmp Master Mix, 40 reactions
TaqMan® PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions
Features
- Analyze mRNA from precious samples such as laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded (FFPE) tissues
- Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan® Gene Expression assays
- For Use With (Equipment): 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System•
- Analyze up to 100 gene expression targets with minimal hands-on time
Preserves Equilibrium of Targets Without Preamplification Bias
- In the past, preamplification kits and techniques often resulted in uneven amplification of targets and incorrect or biased data (as shown by low correlation coefficients between preamplified and unamplified samples). TaqMan® PreAmp Master Mix amplifies with no bias and provides extremely high correlation between amplified and unamplified cDNA
Easy Workflow
- With TaqMan® PreAmp Master Mix, preamplification setup takes only a few minutes. After a one-time pooling of the TaqMan® assays , preamplification setup (mixing the pooled assays with cDNA sample and TaqMan® PreAmp Master Mix) takes only five additional minutes. Cycling takes 1.5 hours using a GeneAmp® 9700 thermal cycler
- GC-Rich PCR Performance: High
- Hot Start: Built-In Hot Start
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