Applied Biosystems™ TaqMan™ Protein Assays Core Reagents Base Kit, 100 Reactions

Applied Biosystems™ TaqMan™ Protein Assays Core Reagents Base Kit, 100 Reactions

TaqMan® Protein Assays Core Reagents Base Kit contains the reagents required to perform the ligation and PCR amplification steps of TaqMan® protein expression experiments. The Universal PCR Assay contains optimized concentrations of TaqMan® probe and oligonucleotide primers (forward and reverse) that are designed to amplify a region of the protease-treated ligation product for detection on an Applied Biosystems real-time PCR system.

For use with equipment: 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOnePlus™, Fast Mode

Contents

• DNA Ligase, 500X (1 × 10 µL)
• Ligase Dilution Buffer, 1X (1 × 1.5 mL)
• Ligation Reaction Buffer, 20X (1 × 0.7 mL)
• Phosphate Buffered Saline (PBS), 1X, pH 7.4 (1 × 1 mL)
• Protease, 100X (1 × 10 µL)
• Universal PCR Assay, 20X (1 × 120 µL)
• Store all components at -15 to -25°C.