Applied Biosystems™ TaqMan™ Protein Assays Core Reagents Base Kit, 100 Reactions
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Order number :
4405460
Applied Biosystems™ TaqMan™ Protein Assays Core Reagents Base Kit, 100 Reactions
TaqMan® Protein Assays Core Reagents Base Kit contains the reagents required to perform the ligation and PCR amplification steps of TaqMan® protein expression experiments. The Universal PCR Assay contains optimized concentrations of TaqMan® probe and oligonucleotide primers (forward and reverse) that are designed to amplify a region of the protease-treated ligation product for detection on an Applied Biosystems real-time PCR system.
For use with equipment: 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOnePlus™, Fast Mode
Contents
- DNA Ligase, 500X (1 × 10 µL)
- Ligase Dilution Buffer, 1X (1 × 1.5 mL)
- Ligation Reaction Buffer, 20X (1 × 0.7 mL)
- Phosphate Buffered Saline (PBS), 1X, pH 7.4 (1 × 1 mL)
- Protease, 100X (1 × 10 µL)
- Universal PCR Assay, 20X (1 × 120 µL)
- Store all components at -15 to -25°C.
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