Applied Biosystems™ TaqMan™ Universal Extraction Control Organism
On demandApplied Biosystems™ TaqMan™ Universal Extraction Control Organism
The
TaqMan Universal Extraction Control Organism contains three vials of
lyophilized Bacillus atrophaeus for use as a process control for cell
lysis and nucleic acid recovery. This control is used with the
proprietary TaqMan assay for Bacillus atrophaeus (Assay ID Ba06596576_s1). Like other gram-positive bacteria, Bacillus atrophaeus
has thick cell walls than can be difficult to lyse. This characteristic
makes gram-positive bacteria an ideal control to monitor the efficiency
of cell lysis and subsequent nucleic acid recovery.
TaqMan Universal Extraction Control Organism is supplied lyophilized in a quantity of 1 × 109 copies/vial and is reconstituted in 200 µL of 1X PBS, pH 7.4 to a final concentration 5 × 16
copies/µL. During nucleic acid isolation, 10 µL of the control is
processed as a stand-alone sample in a background of universal transport
media. It can be added to the negative extraction control, and may also
be added to one or more test samples at the start of the extraction
process. The control is carried through the remainder of the workflow
with the test samples. It is recommended that at least one stand-alone
control sample is run per extraction plate.
Contents
- 3 vials containing lyophilized Bacillus atrophaeus.
There are no specifications
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