Applied Biosystems™resDNASEQ™ Quantitative Synthetic Vero DNA Kit with PrepSEQ™ Residual DNA Sample Preparation Kit

Applied Biosystems™resDNASEQ™ Quantitative Synthetic Vero DNA Kit with PrepSEQ™ Residual DNA Sample Preparation Kit

The resDNASEQ Quantitative Synthetic Vero DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Vero (African Green Monkey) cells, an expression system commonly used for the production of vaccines. Reliable and rapid, the resDNASEQ system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of Vero cell DNA, typically in less than four hours. This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to final product—whether the sample contains high molecular weight or sheared DNA.

Features

• Highly sensitive quantitation using proven TaqMan real-time qPCR technology
• Consistent performance across the expected range of DNA fragment sizes
• Integrated system includes sample preparation kit, master mix, TaqMan primer/probe mix, and Vero plasmid DNA standard
• Manual and automated sample preparation, optimized for quantitative recovery from complex sample matrices

Contents

resDNASEQ Synthetic Vero DNA Control

• Vero Plasmid DNA Control, 40 µL, store at –15 to –25°C
• DNA Dilution Buffer, 7 mL, store at –15 to –25°C before first use and 2–8°C after first use

resDNASEQ Synthetic Vero DNA Real-Time PCR Reagents

• 2X Environmental Master Mix, 2 x 0.75 mL, store at –15 to –25°C before first use and 2–8°C after first use, protect from light
• 10X Synthetic Vero DNA Assay Mix, 300 µL, store at –15 to –25°C, protect from light
• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2–8°C after first use

PrepSEQ Nucleic Acid Extraction Kit

• Lysis Buffer (2 bottles, 50 mL/bottle)
• Binding Solution/Isopropanol (1 empty bottle)
• Wash Buffer Concentrate (2 bottles, 26 mL/bottle)
• Elution Buffer (1 bottle, 25 mL)
• Proteinase K Buffer (1 bottle, 50 mL)
• Magnetic Particles (2 tubes, 1.5 mL/tube)
• Proteinase K (20 mg/mL) (1 tube, 1.25 mL), store at or below -20°C
• PK Buffer II (1 bottle, 11 mL)
• Store all at room temperature unless otherwise indicated.

PrepSEQ Residual DNA Sample Preparation Kit

• Proteinase K (20 mg/mL) (1 tube, 1.25 mL)
• Yeast tRNA (10 mg/mL) (1 tube, 0.5 mL)
• Glycogen (5 mg/mL) (2 tubes, 1.0 mL/tube)