Applied Biosystems™ ExoSAP-IT™ PCR Product Cleanup

Applied Biosystems™ ExoSAP-IT™ PCR Product Cleanup

ExoSAP-IT reagent is designed for simple, quick PCR cleanup for downstream applications, such as DNA sequencing or Single Nucleotide Polymorphism (SNP) analysis. When PCR amplification is complete, any unconsumed dNTPs and primers remaining in the PCR product mixture will interfere with these methods. ExoSAP-IT removes these contaminants.
ExoSAP-IT is added directly to the PCR product and incubated at 37°C for 15 minutes . After PCR treatment, ExoSAP-IT is inactivated simply by heating to 80°C for 15 minutes.
ExoSAP-IT single-step PCR cleanup utilizes two hydrolytic enzymes, Exonuclease I and Shrimp Alkaline Phosphatase (SAP), together in a specially formulated buffer, to remove unwanted dNTPs and primers from PCR products. Exonuclease I removes residual single-stranded primers and any extraneous single-stranded DNA produced in the PCR. SAP removes the remaining dNTPs from the PCR mixture.

Features

• Conserves PCR samples — 100% recovery of both short and long PCR products
• One tube/one step PCR cleanup — Add ExoSAP-IT reagent directly to PCR product
• Eliminates spin columns — Decreases time and expense while increasing yield
• Removes contaminating primers and dNTPs — No interference in downstream applications
• Scalable — Economical for high-throughput purification
• Simple processing — Robotic-friendly; Replaces beads, filtrations, and plates
• Generates less waste than columns