Applied Biosystems™ HT ExoSAP-IT™ High-Throughput PCR Product Cleanup, 1 Each

Applied Biosystems™ HT ExoSAP-IT™ High-Throughput PCR Product Cleanup, 1 Each

HT ExoSAP-IT High-Throughput PCR Product Cleanup is an alternative formulation of ExoSAP-IT reagent specifically designed for the unique requirements of high-throughput, automated platforms, and multi-channel pipettes. HT ExoSAP-IT reagent has both a longer lifetime at higher temperatures and a decreased viscosity for robotic pipetting.
HT ExoSAP-IT reagent is a mixture of Exonuclease I and Shrimp Alkaline Phosphatase (SAP) which removes excess primers and dNTPs following a PCR reaction in a single incubation. HT ExoSAP-IT reagent possesses greater thermal stability and lower viscosity which provides greater ease-of-use in automated 96- and 384-well formats.

Simple Single-step:

HT ExoSAP-IT reagent requires only one pipetting step and two incubations. Just add HT ExoSAP-IT reagent to the PCR product and within 30 minutes sequencing, SNP analysis, single base extension, or fragment analysis can be performed. Enzymatic removal of excess primers and unincorporated nucleotides occurs in one easy step by using HT ExoSAP-IT reagent in a single tube, 8-tube strip, or 12 strips in a 96-well low skirted PCR plate. Our new formatting makes this an ideal product for use with robotics.

Features

• One-tube PCR cleanup — Add HT ExoSAP-IT reagent directly to PCR product
• Exceptional accuracy — Achieve high quality data even with long read lengths
• 10% sample recovery — No loss of PCR products regardless of the fragment size
• Simple, single-step — Replaces the multiple steps and wasted time inherent with beads or columns
• High-throughput processing — Even faster time to results with a low viscosity formulation, allowing robotic pipetting
• Removes excess primers and dNTPs — Does not interfere with downstream applications sequencing– SNP analysis– single base extension– fragment analysis– in vitro transcription
• Scalable — Treat reaction volumes from 5 micro;L to 5 L
• Convenient packaging — Available in 8-tube strips and 12 strips in a 96-well plate
• Stable at +25deg C for 8 hours — Retains full functional activity and at 45°C is stable for one week