Applied Biosystems™ MagMAX™ FFPE DNA/RNA Ultra Kit

Applied Biosystems™ MagMAX™ FFPE DNA/RNA Ultra Kit

The MagMAX™ FFPE DNA/RNA Ultra Kit is designed for sequential isolation of DNA and RNA from the same formaldehyde- or paraformaldehyde-fixed paraffin-embedded (FFPE) tissue sample. The DNA and RNA are recovered in separate eluates, and both are compatible with a broad range of applications, including real-time PCR and next-generation sequencing. The isolation of RNA and DNA from the same FFPE sample makes this kit an ideal method of sample preparation for Oncomine™ Comprehensive and Oncomine™ Focus assays.

Features

• Flexible design that permits both manual and automated isolation of RNA and DNA
• Minimal requirement of 5 µm section or curl input
• Capable of processing 40 µm FFPE sections or curls; greater than 40 µm sections can be processed with an alternative protocol
• Compatible with targeted RNA and DNA sequencing panels
• Contains Dynabeads™ MyOne™ Silane for consistent isolation of RNA, microRNA, and DNA
• Alternative protocols for the isolation of RNA or DNA only
• Purification Target: Total RNA, miRNA, Total Nucleic Acids, Genomic DNA, mRNA, Transcriptome RNA
• Sample Type: FFPE & Fixed Samples

Contents

• Protease: 1 x 385 µL  
• Protease Digestion Buffer: 1 x 10 mL
• Binding Solution: 1 x 38.5 mL
• Nucleic Acid Binding Beads: 1 x 1.95 mL
• DNA Wash Buffer: 1 x 38.5 mL
• Wash Solution 2: 1 x 210 mL
• Elution Solution: 1 x 5 mL
• RNA Wash Buffer Concentrate: 1 x 115 mL
• DNase: 1 x 1.92 mL
• DNase Buffer: 1 x 960 µL