Applied Biosystems™ PrepSEQ™ 1-2-3 Mycoplasma Nucleic Acid Extraction Kit

Applied Biosystems™ PrepSEQ™ 1-2-3 Mycoplasma Nucleic Acid Extraction Kit

The Applied Biosystems® PrepSEQ™ 1-2-3 Nucleic Acid Extraction Kit rapidly extracts Mycoplasma genomic DNA from 100µl sample volumes of research cell lines, stem cell lines, and cell and tissue therapy samples. In the PrepSEQ™ 1-2-3 Kit protocol, a rapid procedure recovers DNA from total lysate of the test sample using magnetic-bead based technology. The Myco Scan Kit is optimized for use with the PrepSEQ 1-2-3 Kit.
High DNA Recovery Sample Preparation
Optimized for use with the Myco Scan Kit, the PrepSEQ 1-2-3 Nucleic Acid Extraction Kit uses proprietary magnetic bead-based separation technology to extract Mycoplasma DNA from mammalian cell lines. The PrepSEQ 1-2-3 Kit extracts Mycoplasma genomic DNA from research cell lines, stem cell lines, cellular and tissue therapy samples with high efficiency.
The protocol is recommended for use with samples with a starting volume of 100 µl with a maximum cell density of 106 cells. The PrepSEQ 1-2-3 Kit provides an easy to use procedure consisting of 1) RNase treatment 2) Proteinase treatment and 3) DNA extraction using the magnetic bead technology (Figure 1). The kit isolates Mycoplasma genomic DNA with high sensitivity from total lysate of test sample. The PrepSEQ 1-2-3 Kit and the Myco Scan Kit allow routine screening of high-value cell lines using an easy, rapid and sensitive protocol. Figure 2 demonstrates how a sample that was spiked with low levels of Mycoplasma bovoculi DNA was found negative for Mycoplasma contamination using a commercially prepared kit. When using the PrepSEQ 1-2-3 Kit and the Myco Scan Kit, the correct positive call for Mycoplasma contamination was made.

Features

• High recovery of Mycoplasma genomic DNA from mammalian cell culture samples
• Rapid easy to use protocol results in under 2 hours
• Compatible with the Myco Scan Mycoplasma Detection assay
• Protocol available for automated sample preparation
• Prep Scale: 100 µl of mammalian cell culture samples
• Target Organism Class: Mycoplasma spp.
• Final Product Type: Genomic DNA
• Sample Type: Cell Culture Supernatants

Contents

• Cell Fractionation Buffer:  3 × 25 mL
• RNase Cocktail™ Enzyme Mix:  2 × 1.0 mL
• Lysis Buffer:  2 × 50 mL
• Binding Solution Bottle(Isopropanol):  1 empty bottle
• Wash Buffer Concentrate:  2 × 26 mL
• Elution Buffer:  25 mL
• Proteinase K (PK) Buffer:  50 mL
• Magnetic Particles:  2 × 1.5 mL
• Proteinase K, 20 mg/mL:  1.25 mL