Applied Biosystems™ USB™ CycleSeq™ Thermostable DNA Polymerase

Applied Biosystems™ USB™ CycleSeq™ Thermostable DNA Polymerase

USB™ CycleSeq™Thermostable DNA Polymerase is a thermostable, DNA polymerase that is exonuclease-free and incorporates ddNTPs with a marked level of efficiency over standard thermostable DNA polymerases. This allows for more even and easy-to-read sequence band patterns, making sequence anomalies easier to identify.
USB CycleSeq Thermostable DNA Polymerase can be substituted for ThermoSequenase DNA polymerase as both enzymes incorporate modified nucleotides.
CycleSeq DNA Polymerase, like Thermo Sequenase DNA Polymerase, includes Thermoplasma acidophilum inorganic pyrophosphatase (TAP). TAP is thermostable and converts pyrophosphate to orthophosphate, a useful feature during cycle sequencing reactions where pyrophosphates are produced.
USB CycleSeq Thermostable DNA Polymerase is useful for cycle sequencing (Sanger sequencing), as it produces sequence data with uniform band intensities which allows for longer and more accurate sequence reads. It is also useful for primer extension protocols during SNP genotyping.

Features

• Source: Recombinant
• Purity: Greater than 95% pure as determined by SDS-PAGE.
• Concentration: 32 units/µL
• Polymerase: Thermostable DNA Polymerase

Contents

• CycleSeq Dilution Buffer: 1 mL
• 10X CycleSeq Reaction Buffer: 1 mL