Applied Biosystems™ViralSEQ™ Quantitative Sf-Rhabdovirus Kit with PrepSEQ™ Nucleic Acid Sample Prep Kit

ViralSEQ™ Quantitative Sf-Rhabdovirus Kit with PrepSEQ™ Nucleic Acid Sample Prep Kit

The Applied Biosystems ViralSEQ Quantitative Sf-Rhabdovirus Kit is a two-step RT-qPCR assay for the detection of sf-rhabdovirus from the Sf9/Sf21 baculovirus platform used in the development of gene therapies, cell-based vaccines, and similar biotherapeutics. This assay leverages a tagged RT-primer design containing a non-viral sequence to enable specific and accurate quantification of negative-sense genomic sf-rhabdovirus RNA. This allows for a more accurate quantification of true viral count and avoids the overestimation that is usually caused by mRNA in non-strand-specifc assay designs.

This kit is bundled together with sample preparation reagents in the form of a PrepSEQ Nucleic Acid Sample Preparation Kit (Cat. No. A50485), which is optimized for highly efficient RNA recovery from common bioproduction matrics.

Features

• Negative-sense-specific RT-PCR assay design for accurate quantification of Sf-rhabdovirus
• Specific to genomic Sf-rhabdovirus RNA; not impacted by the presence of positive-strand mRNA; no cross-reactivity with unrelated DNA/RNA
• Manual and automated sample preparation with PrepSEQ kit, optimized for quantitative recovery of RNA from common bioproduction sample matrices
• Includes master mix, RT-primer, TaqMan Assay mix, and RNA standard

Contents

Sf-Rhabdovirus RNA PCR Reagents

• 10 x Assay Mix, 275 µL, protect from light
• 25 x RT-PCR Enzyme Mix, 110 µL
• 2 x RT-PCR Buffer, 1.375 mL
• RT Primer, 55 µL
• Nuclease Free Water, 1.0 mL, store at -15°C to -25°C until first use. After first use, store at 2°C to 8°C.
• Store all at -15°C to -25°C unless otherwise indicated.

Sf-Rhabdovirus RNA Control

• Sf-rhabdovirus RNA Control (1.25 x 108 copies/µL), 44 µL
• RNA Dilution Buffer, 7 mL
• Store all at -15°C to -25°C.

PrepSEQ Nucleic Acid Extraction Kit

• Lysis Buffer (2 Bottles, 50 mL/bottle)
• Binding Solution/Isopropanol (1 empty bottle)
• Wash Buffer Concentrate (2 Bottles, 26 mL/bottle)
• Elution Buffer (1 Bottle, 25 mL)
• Proteinase K Buffer(1 Bottle, 50 mL)
• Magnetic Particles (2 Tubes, 1.5 mL/tube), store at 2°C to 8°C
• Proteinase K (20 mg/mL) (1 Tube, 1.25 mL), store at or below -20°C
• Pk. Buffer II (1 Bottle, 11 mL)
• Store all at room temperature unless otherwise indicated.

PrepSEQ REsidual DNA Sample Preparation Kit

• Proteinase K (20 mg/mL) (1 Tube, 1.25 mL)
• Yeast tRNA (10 mg/mL) (1 Tube, 0.5 mL)
• Glycogen (5 mg/mL) (2 Tubes, 1.0 mL/tube)
• Store all at or below -20°C.