Axygen® AxyPrep-96 Blood Genomic DNA Kit

Axygen® AxyPrep-96 Blood Genomic DNA Kit

The AxyPrep 96 Blood Genomic DNA Kit is a High-throughput purification of genomic DNA from whole blood in a 96-well format. This Kit is based upon the efficient release of genomic DNA from anticoagulated whole blood by a special cell lysis and protein-removal buffer in combination with Proteinase K digestion. The genomic DNA is then selectively absorbed onto a special 96-well plate for further purification and desalting. The purified DNA is predominantly greater than or equal to =30 Kb in length, and is suitable for a variety of applications demanding highly purified, high molecular weight genomic DNA, such as Southern blot analysis, PCR, etc. Axygen® AxyPrep-96 Blood Genomic DNA Kit, 4x96 preps per Kit

Features

• Protocols for fresh, frozen anticoagulated blood samples, lymphocytes and cultured cells in a 96-well format
• High yield of up to 10 µg of highly purified gDNA per well
• High molecular weight gDNA product ( =30 Kb)
• Rapid spin protocols
• No alcohol precipitation
• Purified genomic DNA is suitable for many downstream applications

Contents

• Round-well block: 4
• 96-well 1.6 ml growblock: 8
• 96-well DNA plate: 4
• Round-well silicone mat: 4
• BF-400 breathable film: 20
• Proteinase K: 120 mg (9 ml)
• PK Buffer: 10 ml
• Buffer BL: 92 ml
• Buffer W1B concentrate: 84 ml
• Buffer W2 concentrate: 3×72 ml 
• Eluent B: 92 ml