Axygen® AxyPrep Bacterial Genomic DNA Miniprep Kit, 250 preps

Axygen® AxyPrep Bacterial Genomic DNA Miniprep Kit, 250 preps

The AxyPrep Bacterial Genomic DNA is suitable for the rapid isolation of up to 20 mg of genomic DNA from 1.0×109 bacterial cells. Following lysis, separation of the genomic DNA from proteins, polysaccharides and lipids is achieved by a unique phase-partition step. The genomic DNA in the lower phase is selectively bound to a special AxyPrep column for further purification and desalting. The highly purified genomic DNA is predominantly 30 kb in length, and is suitable for a variety of applications demanding highly purified, high molecular weight genomic DNA, such as PCR and Southern blot analysis.Axygen® AxyPrep Bacterial Genomic Miniprep Kit, 250 preps per Kit

Features

• High yield of up to 20 µg of highly purified gram-/+ bacterial genomic DNA per prep
• High molecular weight gDNA product( =30 Kb)
• Rapid spin and vacuum protocols
• No alcohol precipitation
• Purified genomic DNA is suitable for many downstream applications

Contents

• Miniprep column: 250
• Spin-filter: 250
• 2 ml Microfuge tube: 750
• 1.5 ml Microfuge tube: 250
• RNase A: 100 μl
• Lysozyme: 2×140 mg
• Buffer S: 50 ml
• 0.25 M EDTA: 10 ml
• Buffer G-A: 130 ml
• Buffer G-B: 125 ml
• Buffer DV (empty): 1
• Buffer DV-A: 10 ml
• Buffer BV: 125 ml
• Buffer W1: 135 ml
• Buffer W2 concentrate: 2×72 ml
• Eluent: 60 ml