Axygen® AxyPrep Bacterial Genomic DNA Miniprep Kit, 50 preps
On demand
Order number :
AP-MN-BT-GDNA-50
Axygen® AxyPrep Bacterial Genomic DNA Miniprep Kit, 50 preps
The AxyPrep Bacterial Genomic DNA is suitable for the rapid isolation of up to 20 mg of genomic DNA from 1.0×109 bacterial cells. Following lysis, separation of the genomic DNA from proteins, polysaccharides and lipids is achieved by a unique phase-partition step. The genomic DNA in the lower phase is selectively bound to a special AxyPrep column for further purification and desalting. The highly purified genomic DNA is predominantly 30 kb in length, and is suitable for a variety of applications demanding highly purified, high molecular weight genomic DNA, such as PCR and Southern blot analysis.Axygen® AxyPrep Bacterial Genomic Miniprep Kit, 50 preps per Kit
Features
- High yield of up to 20 µg of highly purified gram-/+ bacterial genomic DNA per prep
- High molecular weight gDNA product( =30 Kb)
- Rapid spin and vacuum protocols
- No alcohol precipitation
- Purified genomic DNA is suitable for many downstream applications
Contents
- Miniprep column: 50
- Spin-filter: 50
- 2 ml Microfuge tube: 150
- 1.5 ml Microfuge tube: 50
- RNase A: 20 μl
- Lysozyme: 60 mg
- Buffer S: 10 ml
- 0.25 M EDTA: 2 ml
- Buffer G-A: 26 ml
- Buffer G-B: 26 ml
- Buffer DV (empty): 1
- Buffer DV-A: 5 ml
- Buffer BV: 25 ml
- Buffer W1: 28 ml
- Buffer W2 concentrate: 24 ml
- Eluent: 12 ml
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