Axygen® AxyPrep Blood Genomic DNA Midiprep Kit, 10 preps

Axygen® AxyPrep Blood Genomic DNA Midiprep Kit

The AxyPrep Blood Genomic DNA Midiprep and Maxiprep Kits are designed to produce up to 100 µg and 250 µg of high molecular weight genomic DNA respectively from anticoagulated human or animal blood. Purification is based upon a unique phase-partitioning technology in combination with the selective binding of the genomic DNA to a special AxyPrep column. The purified genomic DNA is greater than or equal to 30 Kb in size, and is particularly well suited for PCR, Southern blotting and other applications requiring highly purified, high molecular weight genomic DNA. A vacuum manifold and vacuum source are required.Axygen® AxyPrep Blood Genomic DNA Midiprep Kit, 10 preps per Kit

The genomic DNA produced by this procedure will also include mitochondrial DNA and viral DNA

Features

• Rapid protocols for fresh, frozen anticoagulated blood samples
• Unique phase-partition step for higher yield
• No alcohol precipitation
• High molecular weight gDNA suitable for many downstream applications

Contents

• Midiprep column: 10
• Column adapter: 10
• 1.5 ml Microfuge tube: 20
• Plastic wrench: 1
• Buffer VL: 72 ml
• Buffer G-B: 72 ml
• Buffer DV bottle (empty): 1
• Buffer DV-A: 5 ml
• Buffer BV: 72 ml
• Buffer W1: 96 ml
• Buffer W2 concentrate: 36 ml
• Eluent: 10 ml