Axygen® AxyPrep Blood Genomic DNA Midiprep Kit, 25 preps
On demandAxygen® AxyPrep Blood Genomic DNA Midiprep Kit
The AxyPrep Blood Genomic DNA Midiprep and Maxiprep Kits are designed to produce up to 100 µg and 250 µg of high molecular weight genomic DNA respectively from anticoagulated human or animal blood. Purification is based upon a unique phase-partitioning technology in combination with the selective binding of the genomic DNA to a special AxyPrep column. The purified genomic DNA is greater than or equal to 30 Kb in size, and is particularly well suited for PCR, Southern blotting and other applications requiring highly purified, high molecular weight genomic DNA. A vacuum manifold and vacuum source are required.Axygen® AxyPrep Blood Genomic DNA Midiprep Kit, 25 preps per Kit
The genomic DNA produced by this procedure will also include mitochondrial DNA and viral DNA
Features
- Rapid protocols for fresh, frozen anticoagulated blood samples
- Unique phase-partition step for higher yield
- No alcohol precipitation
- High molecular weight gDNA suitable for many downstream applications
Contents
- Midiprep column: 25
- Column adapter: 25
- 1.5 ml Microfuge tube: 50
- Plastic wrench: 1
- Buffer VL: 180 ml
- Buffer G-B: 180 ml
- Buffer DV bottle (empty): 1
- Buffer DV-A: 10 ml
- Buffer BV: 180 ml
- Buffer W1: 2 x 120 ml
- Buffer W2 concentrate: 2 x 72 ml
- Eluent: 20 ml
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