Axygen® AxyPrep PCR Clean-Up Kit, 50 preps

Axygen® AxyPrep PCR Clean-Up Kit, 50 preps

The AxyPrep PCR Cleanup Kit employs a special Binding Solution in combination with an AxyPrep spin column to achieve high selectivity and recovery of DNA fragments. This product is designed to purify DNA fragments > 75 bp from PCRs and other enzymatic reactions, with an expected recovery of 70-90%. It is not necessary to remove mineral oil overlays from the PCRs before purification. This protocol will remove unincorporated primers (<50 not), enzymes and unlabeled and fluorescent dye-labeled mononucleotides. The purified DNA fragments are suitable for a variety of applications, such as sequencing, ligation, restriction analysis, in vitro transcription, etc. Axygen® AxyPrep PCR Cleanup Kit, 50 preps per Kit

Features

• Rapid spin and vacuum protocols
• Efficient purification of 75 bp-10 Kb amplicons
• No alcohol precipitation
• Up to 90% recovery
• Removal of unincorporated primers <50 not
• Maintains integrity of DNA fragments
• Purified DNA fragments are suitable for many downstream applications

Contents

• PCR column: 50
• 1.5 ml Microfuge tube: 50
• 2 ml Microfuge tube: 50
• Buffer PCR-A: 20 ml
• Buffer W2 concentrate: 24 ml
• Eluent: 5 ml