BD™ Cytometric Bead Array (CBA) Mouse IL-23 p19/p40 Flex Set
The BD™ CBA Mouse IL-23 (p19/p40) Flex Set is a bead-based immunoassay capable of measuring mouse Interleukin-23 (p19/p40) in cell culture supernatant samples. Investigators are encouraged to titrate cell culture supernatant samples, such as with a 1:2 dilution with CBA Assay Diluent, prior to testing. The BD™ CBA Mouse IL-23 (p19/p40) Flex Set is not recommended for testing mouse serum or plasma samples. Mouse reactivity was determined by testing samples with the BD CBA Mouse IL-23 (p19/p40) Flex Set. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Mouse/Rat Soluble Protein Master Buffer Kit (Cat. No. 558266 or 558267).
Preparation, This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 mL polypropylene tube for reconstitution. When reconstituted in 4.0 mL Assay Diluent, the standard has a protein concentration of 2,500 pg/mL.
Recommended Assay Procedures, The BD CBA Mouse IL-23 (p19/p40) Flex Set must be used in conjunction with a BD CBA Mouse/Rat Soluble Protein Master Buffer Kit (Cat. No. 558266, 100 tests, or 558267, 500 tests), a flow cytometer, and FCAP Array™ Software. Detailed instructions for the use of this product can be found in the manual for the BD CBA Mouse/Rat Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Mouse IL-23 (p19/p40) Flex Set will be 2,500 pg/mL.
Assay Range: 10-2,500 pg/mL
Bead Position: D7
Contents
- Mouse IL-23 p19/p40 Standard
- Mouse IL-23 p19/p40 PE Detection Reagent
- Mouse IL-23 p19/p40 Capture Bead D7: 100 Tests (1 ea)
