BD™ Cytometric Bead Array (CBA) Rat IL-1α Flex Set
The BD™ CBA Rat IL-1α Flex Set is a bead-based immunoassay capable of measuring rat interleukin-1α (IL-1α) in serum and cell culture supernatant samples. Rat reactivity was determined by testing samples with the BD CBA Rat IL-1α Flex Set. The biology and function of IL-1α has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Mouse/Rat Soluble Protein Master Buffer Kit (Cat. No. 558266 or 558267).
Preparation, This BD™ CBA Flex Set contains one vial each of Capture Bead and PE Detection Reagent and two vials of Standard. The Capture Bead and PE Detection Reagent components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. The Standard component is lyophilized and should be transferred to a 15 mL polypropylene tube for reconstitution. When reconstituted in 4.0 mL Assay Diluent, the standard has a protein concentration of 10,000 pg/mL.
Recommended Assay Procedures, The BD CBA Rat IL-1α Flex Set must be used in conjunction with a BD CBA Mouse/Rat Soluble Protein Master Buffer Kit (Cat. No. 558266, 100 tests, or 558267, 500 tests), a flow cytometer, and FCAP Array™ Software. Detailed instructions on the use of this product can be found in the manual for the BD CBA Mouse/Rat Soluble Protein Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the BD CBA Rat IL-1α Flex Set will be 10,000 pg/mL.
Assay Range: 40-10,000 pg/mL
Bead Position: A4
Contents
- Rat IL-1α Standard
- Rat IL-1α PE Detection Reagent: 100 Tests (1 ea)
- Rat IL-1α Capture Bead A4: 100 Tests (1 ea)
