BIOBASE™ Fluorescent Quantitative PCR Detection System, MA-6000

BIOBASE™ Fluorescent Quantitative PCR Detection System, MA-6000

Real- time PCR is used for sensitive, specific detection and quantification of nucleic acid targets. We have developed powerful assay design algorithms, optimized qPCR regent, intuitive data analysis software, and flexible instrumentation to help harness the power of qPCR across a rich and diverse set of applications. Explore our robust solutions for your qPCR-based research.

Application: It can be widely used for Infectious disease research, Food pathogen detection, Waterborne pathogen detection, Pharmaceutical analytics, Stem cell research, Pharmacogenomics research, Oncology and genetic disease research, Plant sciences and agricultural biotechnology.

Working principle: The temperature step change is controlled by the semiconductor peltier to realize PCR amplification. Use high-sensitivity MPPC unit to detect fluorescence; Program control channel switching, non-contact excitation/detection on the top structure, coordinated with motor control x and Y axis movement to achieve 96-hole scanning. Finally, accurate analysis is carried out through powerful software.

The integrated design of the scanning module and the heating cover module, relying on its own gravity to compress the heating plate and the reagent cover, and is supported by four compression springs to prevent the sample tube from being crushed; at the same time, the rubber pad around the heating cover is pressed to ensure that there is no external light source interference in the detection ; The bottom of the cam mechanism is used to support the spacing to ensure the smooth sliding of the heating module; the guide rail mechanism on both sides of the heating module prevents the module from shifting and ensures the accuracy of the mechanical scanning structure.

The software includes functions such as absolute quantification experiment, melting curve experiment, relative quantification (AACT) experiment, and genotyping experiment. Enter the attribute setting interface and select different function modules. Guided flow operation, convenient for users to quickly complete experimental settings. The software can open the recent experiment record template for easy viewing of recent experiments and the creation of new experiments.

Temperature control module: On the basis of the six independent temperature control technology, using the enclosed liquid heat transfer efficiency, combined with environmental scanning monitoring regulation auxiliary infrared with heating and intelligent variable frequency system voltage, current, ensures the module heating speed, effectively prevents temperature overshoot no edge effect and the evaporation temperature, not only saves your precious time to amplify the required temperature uniformity and the repeatability of the results achieved perfection, no matter what kind of test mode, application, it can get good data uniformity and repeatability.

Optical module: On behalf of the world's leading optical transmission and collection technology, use optical fiber after the excitation light source for reaction system and the emission of light from the reaction system after excitation, the energy attenuation without conduction to each reaction holes and cold CCD, from physical properties to ensure consistency and authenticity of the excitation and detection, and greatly improve the detection sensitivity, can make your low effective detection and distinguish easily copy sample. The channel mismatch function is added to extend the application field of qPCR to the protein level and provide a new way for the construction of multiple systems of diagnostic reagents.

Channel combination: 6-color excitation light filter and 6-color detection light filter can detect≥21 different fluorescence spectra, channels are completely open, supporting third-party reagent optimization Support FRET application.

Multichannel static fluorescence function: analysis of starting template and end product.

Filter can be customized according to customer need

Software platform: Real-time monitoring, automatic discrimination and calculation of positive and negative results, automatic establishment of standard curve, absolute/relative quantitative, multiple quantitative, fusion curve, gene mutation, quality control graphic analysis, fluorescence correction, PCR amplification efficiency, high-resolution fusion curve (optional match), etc.

Features

• Six independent temperature control
• Thermal compensation technology of temperature module
• No edge effect of temperature and optics have
• Maintenance-free long - life continuous spectral excitation source
• Synchronous and fast acquisition of low temperature CCD 0.15s in cold state
• Relative quantitative high sensitivity 1.5 times effective discrimination
• Flexible, open and user-friendly software platform
• Open reagent consumable platform