CaptureSelect™ CaptureSelect™ CH1-XL RoboColumn, 200 µL

CaptureSelect™ CaptureSelect™ CH1-XL RoboColumn, 200 µL

CaptureSelect RoboColumn™ small chromatography columns are used for fully automated and parallel chromatographic separations on robotic liquid handling workstations.

CaptureSelect CH1-XL Affinity Matrix recognizes the CH1 domain of human IgG antibodies, thereby enabling purification of all Fab fragments, independent of the light-chain type (kappa or lambda) and without binding of over-expressed light chain and light-chain dimers. In addition, it can be used for the purification of human IgGs from all four subclasses (1, 2, 3, and 4).

Features

• Platform approach for purification of high quality and correctly assembled human Fab fragments
• No co-purification of over-expressed free light chains due to selectivity for the CH1 domain
• Elution possible at pH 4.0–4.5 with 50 mM acetate buffer
• Caustic cleaning with up to 50 mM NaOH
• Excellent scalability
• No cross-binding to bovine IgG, therefore presence of fetal bovine serum is not a problem
• Non-animal-derived