Certest™ VIASURE HLA Celiac Real Time PCR Detection Kit 12 x 8-well strips, High Profile
On demandCertest™ VIASURE HLA Celiac Real Time PCR Detection Kit 12 x 8-well strips, High Profile
VIASURE HLA Celiac Real Time PCR Detection Kit is a real-time RT-PCR multiplexed test designed for the simultaneous qualitative detection and differentiation of the main HLA alleles (DQA1*05, DQB1*03:02, DQB1*02, DQA1*02, DQA1*03 and other alleles DQB1 different from DQB1*02) associated with celiac disease (CD) in blood samples.
This test is intended to be used as an aid in the detection of a genetic predisposition for celiac disease based on genotype identified.
The absence of Celiac Disease-associated HLA-DQ2.5, HLA-DQ2.2 and HLA-DQ8 risk factors is an important exclusion criterion for CD. For the HLA-DQB1*02 allele, it can determine the status of a sample as homozygous or heterozygous.
DNA is extracted from blood specimens, multiplied using Real Time amplification and detected using specific primers and a fluorescent reporter dye probe for HLA alleles.
Contents
- HLA Celiac 8-well strips: A mix of enzymes, primers-probes, buffer, dNTPs, stabilizers and Internal control in stabilized format, 6/12 x 8-well strip
- Rehydration Buffer: Solution to reconstitute the stabilized product, 1 vial x 1.8 mL
- HLA Celiac Positive Control: Non-infectious synthetic lyophilized cDNA, 1 vial
- Negative Control: Non template control, 1 vial x 1 mL
- Water RNAse/DNAse free: Water RNAse/DNAse free, 1 vial x 1 mL
- Tear-off 8-cap strips: Optical caps for sealing Wells during thermal cycling, 6/12 x 8-cap strip
- Shell Frame Grid: Shell Frame Grid, 1 or 2
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