Chromo AtTaq DNA Polymerase (Hot Start), 200u

Chromo AtTaq DNA Polymerase (Hot Start), 200u

Chromo AtTaq DNA Polymerase is a complex of specific anti-Taq monoclonal antibody with top quality thermostable Taq DNA Polymerase for automatic "Hot Start" amplification, resulting in greatly enhanced amplification specificity, sensitivity and yield. AtTaq DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5' to 3' direction with the presence of Mg2+ and has the 5' to 3' exonuclease activity. The enzyme is supplemented with inert color tracer dyes.

Features

• Color tracer dyes for ease of visualization of the addition of polymerase to the reaction.
• Use of 1u in every 25μl final reaction allows direct loading and serves as tracking dyes during gel electrophoresis. The blue and pink color dyes migrate approximately at 4kb and 0.3kb respectively on 1% TAE agarose gel.
• Ultra pure recombinant protein which is reversibly complex with anti-Taq monoclonal antibody that blocks replication activity of the enzyme at moderate temperatures.
• Carefully selected anti-Taq antibodies have high thermal stability, providing protection against non-specific primer extension from room temperature to 70°C.
• Formation of complexes between Taq DNA Polymerase and an anti-Taq antibody forms a basis for automatic "Hot Start" amplification, which allows for the assembly of amplification reactions at room temperature.
• High stability of the complexes allows for the enormous increase in amplification specificity, sensitivity and yield in comparison to the conventional amplification assembly method
• Increased specificity as a result of reduced amplification artefacts such as primer-dimer formation and mispriming in multiplex amplification.
• 10X ViBuffer S provided for amplification of more than 5kb amplicon.

Contents

• 200u of AtTaq DNA Polymerase (Hot Start).
• 2ml of 10X ViBuffer A.
• 1ml of 10X ViBuffer S.
• 1ml of 50mM MgCl2.