Chromo MaxTaq DNA Polymerase, 500u

Chromo MaxTaq DNA Polymerase, 500u

Chromo MaxTaq DNA Polymerase is a modified and optimized thermostable enzyme blend containing Taq DNA Polymerase, Pfu DNA Polymerase and enhancing factors. It exhibits the 3' to 5' proofreading activity, resulting in considerably higher amplification fidelity than possible with unmodified Taq DNA Polymerase. The enzyme is supplemented with inert color tracer dyes. Recommended for use in amplification to obtain DNA products up to 20kb.

Features

• Color tracer dyes for ease of visualization of the addition of polymerase to the reaction.
• Use of 1u in every 25μl final reaction allows direct loading and serves as tracking dyes during gel electrophoresis. The blue and pink color dyes migrate approximately at 4kb and 0.3kb respectively on 1% TAE agarose gel.
• Ultra pure recombinant protein allows amplification up to 20kb.
• 10X ViBuffer S provided for amplification of more than 5kb amplicon.
• Excellent for multiplex amplification as it exhibits wider tolerance for Mg2+ and salt concentrations.
• Improves amplification results with critical templates, such as those containing GC-rich regions, palindromes or multiple repeats.
• Increased amplification product yields and purity.
• Generates a mixture of blunt end and 3' dA overhang amplification products, majority of the products are blunt ended.

Contents

• Chromo MaxTaq DNA Polymerase (500u, 1u/μl).
• 10X ViBuffer A (without MgCl2).
• 10X ViBuffer S.
• 50mM MgCl2.