Chromo Taq DNA Polymerase (Thermus aquaticus), 500u

Chromo Taq DNA Polymerase (Thermus aquaticus), 500u

Chromo Taq DNA Polymerase is a thermostable DNA polymerase. It is suitable for applications requiring high temperature synthesis of DNA. Taq DNA Polymerase catalyzed the polymerization of nucleotides into duplex DNA in the 5' to 3' direction with the presence of MgCl2 and has the 5' to 3' exonuclease activity. The enzyme is supplemented with inert color tracer dyes.

Features

• Color tracer dyes for ease of visualization of the addition of polymerase to the reaction.
• Use of 1u in every 25μl final reaction allows direct loading and serves as tracking dyes during gel electrophoresis. The blue and pink color dyes migrate approximately at 4kb and 0.3kb respectively on 1% TAE agarose gel.
• Ultra pure recombinant protein allows amplification up to 20kb.
• 10X ViBuffer S provided for amplification of more than 5kb amplicon.
• Generates mostly 3' dA overhang amplification products which are suitable for TA cloning.

Contents

• Chromo Taq DNA Polymerase (Thermus aquaticus), 500u.
• 10X ViBuffer A (without MgCl2).
• 10X ViBuffer S.
• 50mM MgCl2.