Corning® 1 mg Laminin, Mouse

Corning® 1 mg Laminin, Mouse

Corning® Laminin, mouse, 1mg, is used as a thin coating on tissue-culture surfaces or as a soluble additive to culture medium. Laminin is a major component of basement membranes. It has numerous biological activities including promotion of cell adhesion, migration, chemotaxis, growth, and differentiation, including neurite outgrowth.

Quality:

• Purity >90% by SDS-PAGE (contains entactin)
• Tested for ability to initiate neurite outgrowth of NG-108 human neuroblastoma cells
• Found negative for bacteria, fungi, and mycoplasma

Formulation, Frozen in 0.05 M Tris-HCl, 0.15 M NaCl, pH 7.4.

Molecular Weight, 900 kD (unreduced).

Preparation and Storage, Stable for at least three months at -70°C.

Handling, Recommended concentration: 1-10 µg/cm² of growth surface depending on cell type.