Corning® 100 mL Nu-Serum™ IV Growth Medium Supplement

Corning® 100 mL Nu-Serum™ IV Growth Medium Supplement

Corning® Nu-Serum™ IV Growth Medium Supplement, 100 mL, is a low-protein alternative to newborn calf, fetal bovine, and other sera routinely used for cell culture.

Preparation and Storage, Stable for at least three months at -20°C

Tip, Replaces fetal bovine and other sera on an equivalent volume basis

Quality:

• Tested at a concentration of 10% for the ability to stimulate a =100 fold increase in growth of BHK-21 and a = 13-fold increase in growth of BALB/c-3T3 cells
• Tested for total protein content, pH, and osmolarity (information included with product)
• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi, mycoplasma, and viruses (bovine diarrhea virus, bovine parvovirus, bovine adenovirus, reovirus, and rabies virus); also tested for the absence of cytopathic effects (CPE), inclusion bodies and hemadsorption
• Tested for endotoxin (LAL assay)

Formulation, Corning Nu-Serum™ IV - Frozen solution containing 25% fetal bovine serum, EGF, ECGS, insulin, human transferrin, triiodothyronine, progesterone, estradiol-17ß, testosterone, hydrocortisone, selenous acid, o-phosphorylethanolamine, glucose, amino acids, vitamins, and other trace elements and nutrients contained in the Ham's F12 medium base.

Storage, Shelf life: 3 months from date of shipment at -20°C