Corning® 15mg Endothelial Cell Growth Supplement (ECGS)

Corning® 15mg Endothelial Cell Growth Supplement (ECGS)

Corning® Endothelial Cell Growth Supplement (ECGS), 15mg, is a broadly used supplement to culture a variety of cells, particularly endothelial cells. ECGS contains various growth factors (e.g., acidic FGF or ECGF-a).

Source, Bovine brain.

Quality:

• Tested for ability to promote proliferation of Fetal Bovine Heart Endothelial Cells (FBHEC) in medium containing 10% newborn calf serum
• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi and mycoplasma

Preparation and Storage, Stable for at least three months at 2° to 8°C (lyophilized) or = one month at -20°C (solubilized). Avoid multiple freeze thaws.

Tip, Recommended concentration - 50-150 µg/ mL of medium depending on cell type. Some primary cells require supplementation of 1-100 µg/ mL heparin.

Formulation, Lyophilized from NaCl-containing buffer with streptomycin sulfate; reconstitute in serum-free medium.

Storage, Shelf life: 3 months from date of shipment at 2-8°C (lyophilized) or >= one month at -20°C (solubilized).