Corning® Epidermal Growth Factor (EGF), Mouse Natural (Receptor Grade), 500µg

Corning® Epidermal Growth Factor (EGF), Mouse Natural (Receptor Grade), 500µg

Corning® Epidermal Growth Factor (EGF), mouse natural (receptor grade),500µg, is a low-molecular-weight mitogenic protein that stimulates proliferation of a wide variety of cell types in vitro. 100µg/container, 5/pack. These growth factors have been used to promote long-term proliferation in cell culture and to induce cell differentiation in a variety of cell types under serum-free or reduced serum conditions.

Formulation, Lyophilized from ammonium acetate, carrier-free; reconstitute in dH2O

Preparation and Storage, Stable for at least three months at 2° to 8°C (lyophilized) or =one month solubilized at -20°C . Do not store in frost-free freezer. Avoid multiple freeze/thaws.

Quality:

• Purity: >98% by SDS-PAGE and pH 9.9 PAGE
• Tested for ability to stimulate proliferation of human foreskin fibroblasts in serum-containing medium
• Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi, and mycoplasma

Source, Male mouse submaxillary glands.

Molecular Weight, 6.1 kD.

Purification, EGF, mouse natural (receptor grade) - Purified by BioGel® P10/DEAE chromatography

Tip, Recommended concentration: Approximately 5 ng/ mL medium depending on cell type

Storage, Shelf life: 3 months from date of shipment at 2-8°C (lyophilized) or >= one month at -20°C (solubilized)